shRNA:Article Title: Dipeptide species regulate p38MAPK–Smad3 signalling to maintain chronic myelogenous leukaemia stem cells
Article Snippet: .. Third-generation HuSH shRNA lentiviral vectors based on pGFP-C-shLenti and carrying 29-mer shRNA sequences targetting the mouse Slc15A2 gene (TL503323B; mouse Slc15A2 shRNA B: 5′-GAACCGTTCTGAGGACATTCCAAAGCGAC-3′, and TL503323D; mouse Slc15A2 shRNA D: 5′-TATCGGCTGATCTCCAAGTGCGGAGTTAA-3′), as well as control scrambled shRNA, were purchased from Origene (Rockville, MD). pCMV-VSV-G and pCMV-dR8.2 dvpr were provided by Addgene (Cambridge, MA). .. 293TN producer cells (purchased from System Biosciences; Mountain View, CA) were transiently transfected with pGFP-C-shLenti vector (6 μg per 100-mm plate), pCMV-VSV-G (1.5 μg) and pCMV-dR8.2 dvpr (4.5 μg) using FuGene6 (Roche) as described above for retroviral transduction of KLS + cells.
Article Title: Composition for inhibiting growth or proliferation of chronic myelogenous leukemia cancer stem cells, and screening method therefor
Article Snippet: .. Short Hairpin (Sh) RNA Targeting Slc15A2 mRNA Third-generation HuSH shRNA lentiviral vectors based on pGFP-C-shLenti and carrying 29-mer shRNA sequences targeting mouse Slc15A2 gene (mouse Slc15A2 shB: 5′-GAA CCG TTC TGA GGA CAT TCC AAA GCG AC-3′ mouse Slc15A2 shD: 5′-TAT CGG CTG ATC TCC AAG TGC GGA GTT AA-3′) and control scrambled shRNA were purchased from Origene (Rockville, Md.). pCMV-VSV-G and pCMV-dR8.2 dvpr were provided by Addgene (Cambridge, Mass.). .. 293TN producer cells (System Biosciences; Mountain View, Calif.) were transiently transfected with pGFP-C-shLenti vector (6 μg per 100 mm plate), pCMV-VSV-G (1.5 μg) and pCMV-dR8.2 dvpr (4.5 μg) using FuGene6 (Roche) as described above for retroviral transduction of KLS+ cells.
Article Title: Dipeptide species regulate p38MAPK-Smad3 signalling to maintain chronic myelogenous leukaemia stem cells.
Article Snippet: .. Third-generation HuSH shRNA lentiviral vectors based on pGFP-C-shLenti and carrying 29-mer shRNA sequences targetting the mouse Slc15A2 gene (TL503323B; mouse Slc15A2 shRNA B: 50-GAA CCGTTCTGAGGACATTCCAAAGCGAC-30 , and TL503323D; mouse Slc15A2 shRNA D: 50-TATCGGCTGATCTCCAAGTGCGGAGTTAA-30), as well as control scrambled shRNA, were purchased from Origene (Rockville, MD). pCMV-VSV-G and pCMV-dR8.2 dvpr were provided by Addgene (Cambridge, MA). .. 293TN producer cells (purchased from System Biosciences; Mountain View, CA) were transiently transfected with pGFP-C-shLenti vector (6 mg per 100-mm plate), pCMV-VSV-G (1.5 mg) and pCMV-dR8.2 dvpr (4.5 mg) using FuGene6 (Roche) as described above for retroviral transduction of KLSþ cells.
Control:Article Title: Dipeptide species regulate p38MAPK–Smad3 signalling to maintain chronic myelogenous leukaemia stem cells
Article Snippet: .. Third-generation HuSH shRNA lentiviral vectors based on pGFP-C-shLenti and carrying 29-mer shRNA sequences targetting the mouse Slc15A2 gene (TL503323B; mouse Slc15A2 shRNA B: 5′-GAACCGTTCTGAGGACATTCCAAAGCGAC-3′, and TL503323D; mouse Slc15A2 shRNA D: 5′-TATCGGCTGATCTCCAAGTGCGGAGTTAA-3′), as well as control scrambled shRNA, were purchased from Origene (Rockville, MD). pCMV-VSV-G and pCMV-dR8.2 dvpr were provided by Addgene (Cambridge, MA). .. 293TN producer cells (purchased from System Biosciences; Mountain View, CA) were transiently transfected with pGFP-C-shLenti vector (6 μg per 100-mm plate), pCMV-VSV-G (1.5 μg) and pCMV-dR8.2 dvpr (4.5 μg) using FuGene6 (Roche) as described above for retroviral transduction of KLS + cells.
Article Title: Composition for inhibiting growth or proliferation of chronic myelogenous leukemia cancer stem cells, and screening method therefor
Article Snippet: .. Short Hairpin (Sh) RNA Targeting Slc15A2 mRNA Third-generation HuSH shRNA lentiviral vectors based on pGFP-C-shLenti and carrying 29-mer shRNA sequences targeting mouse Slc15A2 gene (mouse Slc15A2 shB: 5′-GAA CCG TTC TGA GGA CAT TCC AAA GCG AC-3′ mouse Slc15A2 shD: 5′-TAT CGG CTG ATC TCC AAG TGC GGA GTT AA-3′) and control scrambled shRNA were purchased from Origene (Rockville, Md.). pCMV-VSV-G and pCMV-dR8.2 dvpr were provided by Addgene (Cambridge, Mass.). .. 293TN producer cells (System Biosciences; Mountain View, Calif.) were transiently transfected with pGFP-C-shLenti vector (6 μg per 100 mm plate), pCMV-VSV-G (1.5 μg) and pCMV-dR8.2 dvpr (4.5 μg) using FuGene6 (Roche) as described above for retroviral transduction of KLS+ cells.
Article Title: Dipeptide species regulate p38MAPK-Smad3 signalling to maintain chronic myelogenous leukaemia stem cells.
Article Snippet: .. Third-generation HuSH shRNA lentiviral vectors based on pGFP-C-shLenti and carrying 29-mer shRNA sequences targetting the mouse Slc15A2 gene (TL503323B; mouse Slc15A2 shRNA B: 50-GAA CCGTTCTGAGGACATTCCAAAGCGAC-30 , and TL503323D; mouse Slc15A2 shRNA D: 50-TATCGGCTGATCTCCAAGTGCGGAGTTAA-30), as well as control scrambled shRNA, were purchased from Origene (Rockville, MD). pCMV-VSV-G and pCMV-dR8.2 dvpr were provided by Addgene (Cambridge, MA). .. 293TN producer cells (purchased from System Biosciences; Mountain View, CA) were transiently transfected with pGFP-C-shLenti vector (6 mg per 100-mm plate), pCMV-VSV-G (1.5 mg) and pCMV-dR8.2 dvpr (4.5 mg) using FuGene6 (Roche) as described above for retroviral transduction of KLSþ cells.
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